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Transformation of alpha‐santonin via two independent pathways by Pseudomonas strain S ATCC 43388
(Oxford University Press, 1993) Colaco, Dilecta; Furtado, I.; Naik, U. P.; Mavinkurve, S.; Paknikar, S. K.
Isolation and Characterization of Listeria Species from Raw and Processed Meats
(Indian Association of Veterinary Public Health Specialists, 2010) Doijad, S.P.; Vaidya, V.; Garg, S.; Kalekar, S.; Rodrigues, J.; D’Costa, Dilecta; Bhosle, S. N.; Barhuddhe, S. B.
A total of 109 meat and meat product samples (50 beef, 52 pork sausages and 7 pork) were examined for the presence of Listeria species. Isolates were confirmed by biochemical characterization and haemolysis on blood agar. A total of 25 (22.94 percent) isolates were recovered. Of these, 7 (6.42 percent) were L. monocytogenes, while one (0.9 percent) was L. ivanovii. Other isolates were L. seeligeri (10), L. innocua (4), L. welshimeri (2) and L. grayi (1). All the isolates were further subjected to a PCR assay for detection of the hlyA gene, the hlyA gene was detected in 7 isolates. Multiplex-PCR serotyping assay revealed three of the strains belonging to serovar group 4b, 4d, 4e, and four to serovar group 1/2a, 3a. The prevalence of L. monocytogenes in raw meat, particularly serogroup 4b, 4d, 4e in present study indicates a potential threat to public health.
Influence of labour welfare facilities on job satisfaction: A study of pharmaceutical companies in Goa
(Indian Society of Labour Economics, 2011) DeSouza, Christina
This paper highlights the influence of labour welfare facilities on the job satisfaction of employees in Indian and multinational pharmaceutical companies in Goa. A stratified proportionate sample of 201 employees was administered the Labour Welfare Inventory by S.K. Srivastava, (2002) and the Job Satisfaction Scale by Dr. Rita Shresthya and H.C. Ganguli, (1994). The eight dimensions of labour welfare (including education/training, housing, subsidised loans, recreation, safety, canteen, medical facilities, and others) were found to be positively and significantly correlated with the job satisfaction of employees in these companies. Regression analysis specified the dimensions of education/training and others, as indicative of influencing the job satisfaction of employees in these pharmaceutical companies. The results also showed that the statutory labour welfare facilities constitute a better predictor of job satisfaction than the non-statutory labour welfare facilities in these companies. Employees in the multinational pharmaceutical companies in Goa reported better provision of labour welfare facilities and were found to experience a higher level of job satisfaction than their fellow mates in Indian pharmaceutical companies.
Genotypic characterization of Listeria monocytogenes isolated from humans in India
(Taylor & Francis, 2011) Kalekar, Sushanta; Rodrigues, Jenney; D'Costa, Dilecta; Doijad, Swapnil; Ashok Kumar, J; Malik, S. V. S; Kalorey, D. R; Hain, T; Chakraborty, T; Barbuddhe, Sukhadeo B.
Listeria monocytogenes is a foodborne pathogen associated with severe diseases in humans and animals. The genotypic analysis of 17 L. monocytogenes isolates recovered from humans in India during 2006–2009 using multiplex serotyping PCR allowing serovar predictions, conventional serology and by pulsed field gel electrophoresis (PFGE) is presented. The isolates were recovered from patients exhibiting various clinical conditions. A multiplex-PCR based serotyping assay revealed 88·24% (15/17) of the strains belonging to the serovar group 4b, 4d, 4e and 11·76% (2/17) to the serovar group 1/2b, 3b. Conventional serology indicated that 13 (76·47%) L. monocytogenes isolates to be of serotype 4b, 2 (11·76%) serotype 4d, and 2 (11·76%) serotype 1/2b. Ten ApaI and nine AscI pulsotypes were recognized among the 17 human isolates. PFGE analysis allowed discrimination among isolates of the same serotype and among isolates from the same sampling areas or those isolated from different areas. Thus, PFGE together with multiplex-PCR serotyping allows rapid discrimination of L. monocytogenes strains. In addition, the predominance of L. monocytogenes serotype 4b is of concern, as this serotype has been most frequently associated with human listeriosis outbreaks.
Incidence and genetic variability of Listeria species from three milk processing plants
(Elsevier, 2011) Doijad, Swapnil; Barbuddhe, S. B.; Garg, Sandeep; Kalekar, Sushanta; Rodrigues, Jenney; D’Costa, Dilecta; Bhosle, S.; Chakraborty, Trinad
The presence of Listeria in three milk processing environments as a potential source of milk contamination was assessed. Swab samples (n = 210) taken from milk processing plants were examined. Sample sites included the milk processing equipment, besides areas handling raw and pasteurized milk. The USDA Listeria-selective enrichment procedure was used to process the samples. Forty one (19.52%) Listeria isolates were recovered. The isolates were further subjected to biochemical and genotypic characterization. Out of 41 isolates, 16 (7.62%) were confirmed as Listeria monocytogenes, 2 (0.95%) as L. ivanovii, 19 (9.05%) as L. innocua. 1 (0.48%) as L. seeligeri and 3 (1.43%) as L. grayi. All the L. monocytogenes isolates were positive for the hlyA gene. PCR based serotyping revealed all L. monocytogenes to be of 1/2a, 1/2c, 3a and 3c serovar group. AscI and ApaI restriction analysis yielded four PFGE clusters for 16 L. monocytogenes isolates obtained from raw milk collector, milk silos, buttermilk mixer, cheese and other milk product processor. No predominant PFGE cluster was observed among these L. monocytogenes isolates. The main sources of L. monocytogenes were found to be raw milk collector and milk silos. In the present study L. monocytogenes was isolated from milk and milk products processing plants which could cross-contaminate the processed products and may possess a potential threat to public health.
Bacteriological Quality of Milk at Different Levels of Collection in Goa
(Indian Association of Veterinary Public Health Specialists, 2011) D’Costa, Dilecta; Bhosle, S. N.; Dhuri, R. B.; Barbuddhe, S. B.
The bacteriological quality of milk at different levels of collection was studied. Samples were collected in sterile containers at quarterly intervals. The samples were analysed for total vibal count, methylene blue reduction test (MBRT) and California mastitis test (CMT). Subclinical mastitis was found in 23.8 percent of the animals. The average methylene blue reduction time decreased from the farm to the processing unit. The average counts were 2.67x104, 1.59x104, 9.23x105 and 2.35x106 cfu/ml at farmers' field, milking utensils, collection centers and receiving dock (processing point), respectively. The milking methods, milk containers and time interval from the collection at the udder level to the receiving dock played a major role in the deterioration of the milk quality along with the climatic conditions prevalent in a particular season.
Characterization and Mössbauer Study of Ni0.45Zn0.55Fe2O4 Nanoparticles Prepared by Novel Precursor Method
(American Institute of Physics, 2011) Kothawale, M. M.; Tangsali, R. B.; Joseph, Jaison; Naik, G. K.; Budkuley, J. S.; Meena, Sher Singh; Yusuf, S. M.; Yakhmi, J. V.
Nanoparticles of were chemically synthesized by novel precursor method at low temperature. Average particles size was found to be 37 nm. The Mössbauer spectroscopy studies were carried out on the sample for the investigation of magnetic and structural arrangement of Fe in the nano‐crystalline ferrite samples. The values of isomer shift are consistent with Fe ions in trivalent state. The low values of the quadrupole splitting identifies that the local symmetry of the magnetic phase of A—sites is close to cubic while that of B—site is close to trigonal. The broad collapsing sextet B1 can be correlated to the presence of relaxation effect in the sample.
Can Gram Sabha Check Corruption at the Grassroots? Study of Goa
(SAGE Publications, 2012) Salgaonkar, Seema P; Salgaonkar, Pradeep
There are certain grey areas in the institutions of local governance, particularly at Panchayat level where corruption is often rampant. Gram Sabha, if vigilant, can act as a watchdog against corruption. Normally people participate in Gram Sabhas to pursue their personal interests, but in this process of pursuance, they also begin to respond to common problems. This study examines the role of Gram Sabha in controlling corruption at grassroots with a case study of Goa.
Amalgam of Leadership styles: A Case Study of First Chief Minister of Goa
(Indian Political Science Association, 2012) Salgaonkar, Seema P
Shri Dayanand Bandodkar, the first Chief Minister of Goa, or simply 'Bhau' of the masses have been an inspiration to the down trodden as he was God incarnate to uplift them from socio-economic misery. He was ray of hope to the state as in his administration Goa developed in leaps and bounds. This paper is an attempt to understand the leadership styles ofBhau which enabled him to rule Goa for more than a decade. Bhau had deep concern for the masses. He worked towards their welfare. Therefore he was considered as a true democrat. However his style of functioning was more autocratic and he was even accused, not only by opposition members but even by his own colleagues, as a 'hukumshah'. The paper unfolds this paradox with the help of various observations.
Prevalence, Serogroups, Shiga-toxin Genes and Pulsed Field Gel Electrophoresis Analyses of Escherichia coli Isolated from Bovine Milk
(Springer Nature, 2013) D’Costa, Dilecta; Bhosle, Saroj N.; Dhuri, R. B.; Doijad, S. P.; Poharkar, K. V.; Kalorey, D. R.; Barbuddhe, S. B.
Shiga toxin-producing Escherichia coli (STEC) including non-O157 strains have been linked to outbreaks and sporadic cases of illness worldwide. A total of 647 milk samples were collected at different levels of collection and processing (udder, milking utensils, milk collection centres and receiving dock) within West Coast region of India. The milk samples were screened for the presence of E. coli and further tested for the Shiga-toxin (stx) genes by PCR. The isolates were characterized for their serogroups and XbaI digestion patterns of total DNA separated by pulsed-field gel electrophoresis (PFGE). A total of 77 (11.90 %) isolates were confirmed as having E. coli. The serogroups reported were O4, O60, O112, O56, O159, O120, O2, O83, O88, O95, O141, O21, O25, O80, O140, O97, O24, O166, O146, O51, O169, O147, O103, O18, O100, O15, O69, O43, O7, O3, O45, O124, O110, O84, and O114. Out of the 77 E. coli isolates, 25 (32.46 %) could be classified as Shiga-toxigenic based on PCR results. Of these 11, 3 and 11 isolates were positive for stx1, stx2, and both stx1 and stx2, respectively. PFGE profiles indicated genetic diversity of E. coli strains. Much variation was observed among isolates recovered at different levels of collection. Further research is needed to uncover unique characteristics and resistance of non-O157 STEC strains.
Comparison of degradation of methylene blue dye by zno, n doped zno and iron ore rejects
(Deuton-X Ltd, 2014) Borker, Vrinda; Mordekar, Rajashri Karmali; Rane, Koyar
Textile effluent containing unused dye when released in surroundings pollutes water bodies. It requires processing before disposal. Iron ore reject created during mining creates environmental pollution but contains minerals of technological importance. It has ~30-50 % iron in the form of γ-Fe2O3, α-Fe2O3 and Fe3O4 is wasted if thrown in fields, so can be used to degrade organic dyes. Mineralization of methylene blue, MB a model dye is carried out using photocatalyst either iron ore reject, synthesized ZnO or ZnO1-xNx and the results are compared. ZnO is synthesised from oxalate and nitrogen doped ZnO from hydrazinated oxalate precursors. FTIR study of zinc complexes indicates formation of precursors and XRD of decomposed complexes reveal formation of zinc oxide with wurtzite structure. The presence of nitrogen in ZnO1-xNx is confirmed by chemical estimation and XPS studies. SEM reveals the particle size of ZnO1-xNx ~69 nm and ZnO ~0.5-1μm. ZnO1-xNx absorbs in the visible region and ZnO in UV region. Band gap energy calculated using Diffuse reflectance Spectrum is 2.48 eV for the former, 3.19eV for the later and 2.38 eV for ore reject. Mineralizing property of ore reject, ZnO and ZnO1-xNx are compared by electrons spray ionisation mass spectrometry study (ESI-MS) of degradation products, COD measurement and CO2, NO3- and SO4-2 estimation. Reusability study, kinetic study of degradation of MB dye using photocatalysts and ESI-MS study of degraded products of MB reveal better efficacy of iron ore rejects amongst three. Thus efficiency of iron ore reject > ZnO1-xNx. > ZnO as photocatalyst.
Magnetic Properties of Textured Nanocrystalline Mn-Zn Ferrite Thin Films Fabricated by Pulsed Laser Deposition.Thin Films Fabricated by Pulsed Laser Deposit
(Natural Sciences Publishing, 2014) Joseph, Jaison; Tangsali, R. B.; Choudhary, R. J.; Phase, D. M.; Ganeshan, V.
MnxZn1-xFe2O4 nanoparticles were chemically synthesized by co- precipitating metal ions in alkaline aqueous solutions. The XRD peaks match up to spinel ferrites without any multi phase indication and clear visibility of ferrite FT-IR absorption bands confirm single phase spinal formation. Particle size derived from XRD data is authenticated by TEM micrographs. Thin films fabricated from this material on quartz substrate by pulse laser deposition were characterised using XRD. The XRD data revealed formation of spinel structure with a reasonable degree of texture. AFM analysis confirms nano granular film morphology with dimensions comparable to that of target grain. Magnetic data obtained from textured nanocrystalline Mn-Zn ferrite thin film measurements made known enhanced coercivity. The observed enhanced coercivity is explained with due consideration of film texture and surface disorder that originated from Mn concentration specific initial adsorption prior to nucleation, resulting in directional film growth.
Prevalence and characterisation of Listeria spp. from seafood
(Indian Council of Agricultural Research, 2015) Rodrigues, Jenney; Kalekar, Sushanta; Doijad, Swapnil; Poharkar, Krupali; D'Costa, Dilecta; Barbuddhe, Sukhadeo B.
Listeria monocytogenes, the causative organism of listeriosis, is primarily transmitted to humans through contaminated food. We examined the prevalence of L. monocytogenes isolates from fishery products marketed in Goa, India. A total of 221 raw seafood samples were examined for the presence of Listeria species following ISO 11290 protocol. Thirty seven (16.74%) samples were positive for Listeria species. Out of these, isolates from 4 (1.8%) samples were confirmed asL. monocytogenes, the remaining 33 isolates were Listeria innocua. All the isolates were subjected to PCR for the hlyAgene, which was detected in all the L. monocytogenes isolates. The isolates were also found to express phosphatidyl-inositol specific phospholipase C activity on ALOA agar. Multiplex PCR-based clonal typing revealed three L. monocytogenesisolates to be of serovar group 1/2a, 1/2c, 3a, 3c while the remaining isolates belonged to serovar group 1/2b, 3b 4b, 4d and 4e. The isolates were grouped into two AscI and ApaI PFGE (pulsed field gel electrophoresis) profiles. Prevalence ofL. monocytogenes in fresh seafood is of significance as it may contaminate and persist in the processing environment.
Influence of T.V advertisements on children's food and beverage preferences: A study from Goa
(World Wide Journals, 2015) Divakar, Radhamani; AnjanaRaju, Guntur
This study was conducted to examine the influence of T.V advertising on food and beverage preferences of children in urban and rural areas of Goa. A sample of 1000 children of 6-12 years of age across Goa was selected. It was found that children in urban as well as rural areas were influenced by television advertisements and purchased most of the advertised food products. Products of Dominos, McDonald and KFC which were expensive and available only in the city outlets had more demand in urban areas as compared to rural areas.
Parental regulation of children's T.V. viewing behaviour
(Dr. Baral's Research Foundation, 2015) Divakar, Radhamani; AnjanaRaju, Guntur
Parents play a major role in children acquiring consumer knowledge and skills through parent - child communication, training and modelling (Ward, Wackman and Wartella 1977). Parents can teach children about T.V advertising by actively participating in children's television viewing (Walsh, Laczniak and Carlson, 1998) and establishing rules to guide children's T.V viewing and processing of television communication (Armstrong and Brucks, 1988). This study is based on the interviews conducted with parent's and focuses on their relationship to children's television viewing, on circumstances where the family watches T.V together, and on discussion about television programmes with children. It covered large area of thought from parent's T.V viewing habits to control exercised, intent explained to child and effect of T.V viewing on children. The sample has been drawn from 10 primary schools in North and South districts of Goa. The questionnaire was filled by one of the parent of each of the 200 children studied . The results of the study revealed that parents in Goa resort to co-viewing and controlling of T.V programmes of children in general. They control by limiting the time of T.V watching and controlling the content of T.V watched by children and making the intent clear to their children. Since parents themselves watch television and advertisements they are able to monitor children's T.V viewing behaviour.
Impact of various factors on children's fast food consumption in Goa
(SLAA Publications Private Limited, 2015) Divakar, Radhamani; AnjanaRaju, Guntur
The objective of this study is to examine the impact of various factors on consumption of fastfood among children in the age group of 6-12 years. The sample size was 1000 children from various schools in urban and rural areas in the State of Goa. From the study it was found that the influence of various factors was higher in rural areas as compared to urban areas. It was found that product characteristics and convenience are the main factors that influence children towards fast food followed by casual attitude for homemadefood.
Characterization of Listeria monocytogenes isolated from human clinical cases
(International Journal of Medical and Health Sciences (IJMHS), 2015) Kalekar, Sushanta; Doijad, Swapnil; Poharkar, Krupali V.; Rodriguez, Savio; Kalorey, D R; Kurkure, Nitin V; Rawool, Deepak B; D'Costa, Dilecta; Bhosle, Saroj; Barbuddhe, S B
Introduction: Listeria monocytogenes is an emerging foodborne pathogen and causative agent of listeriosis. It is one of the cause of spontaneous abortions and meningitis. The objective of the study was to determine the incidences of L. monocytogenes from human cases of spontaneous abortions or having a history of spontaneous abortions and meningitis. Materials and Methods: A total of 481 samples from humans having a history of spontaneous abortions and meningitis were analyzed. Isolation of the pathogen was attempted employing two step enrichment followed by plating on selective media. Characterization of the isolates was based on biochemical tests, haemolysis on blood agar, CAMP test, phosphatidyl inositol-speciï¬ c phospholipase C assay, multiplex PCR to detect virulence-associated genes (hlyA, actA and iap) and serotyping by conventional and PCR based method. Results: Of the 481 samples analysed, 7 (1.45%) samples from abortion cases were found positive, while no CSF sample from meningitis cases was positive for L. monocytogenes. All the isolates showed in-vitro virulence characteristics such as hemolysis on 5% sheep blood agar, positive CAMP test and showed hallow formation on ALOA medium. All the isolates showed the presence of the hly, actA and iap genes. Conventional serotyping as well as PCR based serotyping revealed the isolates to be of 4b, 1/2b and 4e serotypes. Antibiotic sensitivity assay showed that the isolates were sensitive to trimethoprim, however, the isolates were least sensitive to meropenem. Conclusions: The study highlights the incidence of L. monocytogenes in humans with spontaneous abortions. KEYWORDS: Listeria monocytogenes, Serotypes, Isolation, PCR, human.
Synthesis, characterization and Transport properties of CoxZn1-xFe2O4 Nanoparticles
(IOP Publishing Ltd., 2015) Joseph, Jaison; Tangsali, R. B.; Gurav, S. M.
Spinel ferrites are commercially important materials due to their excellent electrical and magnetic properties. Mixed metal spinel ferrites, essentially with low hysteresis loss and small particle sizes, have found new applications in areas like biosensors, drug delivery systems, and hyperthermia. The electrical conductivity, which gives credible information about conduction mechanism, is one of the important properties of ferrites. The order of magnitude of conductivity greatly influences the dielectric and magnetic behavior of the ferrites and depends on the preparative method. The present work deals with investigations into electrical, dielectric and thermo power parameters of CoxZn1-xFe2O4 ferrite nano particles synthesized by co-precipitation method. The effect of variation of the cobalt concentration in the sample on transport properties of nano ferrites has been analyzed.
Microstructure and magnetic properties of patterned nano crystalline zinc ferrite thin film fabricated by pulse laser deposition
(Elsevier, 2015) Joseph, Jaison; Tangsali, R. B.; Mahadevan Pillai, V. P.; Choudhary, R. J.; Phase, D. M.; Ganeshan, V.
Patterned nano crystalline ZnFe2O4 thin film was fabricated on quartz substrate by pulse laser deposition. XRD and Raman spectroscopic techniques were employed for structural characterization of the film. Silencing of a small number of prominent ferrite XRD peaks in thin film signify mild textured film growth. The observed XRD peak position swing with respect to the target material in thin film indicates formation of lateral strain in opposite directions during film growth. The thin film XRD peak position shift with target material data as reference is explained by suggesting an appropriate film growth model. Designated ferrite Raman emission peaks originated from film surface authenticates the stoichiometric and structural stability of ferrite material. AFM images indicate specific pattern formation with nanogranular morphology. Magnetic property measurements of the thin film revealed enhanced properties which are explained on the basis of texture, lattice strain, and surface features that are originated from patterned thin film growth.
Structure and magnetic properties of highly textured nanocrystalline Mn–Zn ferrite thin film
(Elsevier, 2015) Joseph, Jaison; Tangsali, R. B.; Mahadevan Pillai, V. P.; Choudhary, R. J.; Phase, D. M.; Ganeshan, V.
Nanoparticles of Mn0.2Zn0.8Fe2O4 were chemically synthesized by co-precipitating the metal ions in aqueous solutions in a suitable alkaline medium. The identified XRD peaks confirm single phase spinal formation. The nanoparticle size authentication is carried out from XRD data using Debye Scherrer equation. Thin film fabricated from this nanomaterial by pulse laser deposition technique on quartz substrate was characterized using XRD and Raman spectroscopic techniques. XRD results revealed the formation of high degree of texture in the film. AFM analysis confirms nanogranular morphology and preferred directional growth. A high deposition pressure and the use of a laser plume confined to a small area for transportation of the target species created certain level of porosity in the deposited thin film. Magnetic property measurement of this highly textured nanocrystalline Mn–Zn ferrite thin film revealed enhancement in properties, which are explained on the basis of texture and surface features originated from film growth mechanism.

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