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Browsing Faculty Publications by Author "Barbuddhe, S. B."

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    Bacteriological Quality of Milk at Different Levels of Collection in Goa
    (Indian Association of Veterinary Public Health Specialists, 2011) D’Costa, Dilecta; Bhosle, S. N.; Dhuri, R. B.; Barbuddhe, S. B.
    The bacteriological quality of milk at different levels of collection was studied. Samples were collected in sterile containers at quarterly intervals. The samples were analysed for total vibal count, methylene blue reduction test (MBRT) and California mastitis test (CMT). Subclinical mastitis was found in 23.8 percent of the animals. The average methylene blue reduction time decreased from the farm to the processing unit. The average counts were 2.67x104, 1.59x104, 9.23x105 and 2.35x106 cfu/ml at farmers' field, milking utensils, collection centers and receiving dock (processing point), respectively. The milking methods, milk containers and time interval from the collection at the udder level to the receiving dock played a major role in the deterioration of the milk quality along with the climatic conditions prevalent in a particular season.
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    Incidence and genetic variability of Listeria species from three milk processing plants
    (Elsevier, 2011) Doijad, Swapnil; Barbuddhe, S. B.; Garg, Sandeep; Kalekar, Sushanta; Rodrigues, Jenney; D’Costa, Dilecta; Bhosle, S.; Chakraborty, Trinad
    The presence of Listeria in three milk processing environments as a potential source of milk contamination was assessed. Swab samples (n = 210) taken from milk processing plants were examined. Sample sites included the milk processing equipment, besides areas handling raw and pasteurized milk. The USDA Listeria-selective enrichment procedure was used to process the samples. Forty one (19.52%) Listeria isolates were recovered. The isolates were further subjected to biochemical and genotypic characterization. Out of 41 isolates, 16 (7.62%) were confirmed as Listeria monocytogenes, 2 (0.95%) as L. ivanovii, 19 (9.05%) as L. innocua. 1 (0.48%) as L. seeligeri and 3 (1.43%) as L. grayi. All the L. monocytogenes isolates were positive for the hlyA gene. PCR based serotyping revealed all L. monocytogenes to be of 1/2a, 1/2c, 3a and 3c serovar group. AscI and ApaI restriction analysis yielded four PFGE clusters for 16 L. monocytogenes isolates obtained from raw milk collector, milk silos, buttermilk mixer, cheese and other milk product processor. No predominant PFGE cluster was observed among these L. monocytogenes isolates. The main sources of L. monocytogenes were found to be raw milk collector and milk silos. In the present study L. monocytogenes was isolated from milk and milk products processing plants which could cross-contaminate the processed products and may possess a potential threat to public health.
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    Mangrove ecosystems: An adopted habitat for pathogenic Salmonella spp.
    (Water Environment Federation, 2016) Poharkar, Krupali V.; Kerkar, Savita; D’Costa, Dilecta; Doijad, Swapnil; Barbuddhe, S. B.
    Mangroves are affected by industrial and anthropogenic factors. Although mangroves have been widely studied, investigations of pathogens that may affect public health significance are largely lacking even while incidences of diseases linked with the consumption of mangrove-associated food have increased. A total of 150 samples of water, sediment, and biota were collected from ten mangrove ecosystems in Goa, India. Total viable counts of pathogens such as E. coli, Listeria, Salmonella, and Vibrio spp. ranged from 1.25 to 3.9 × 103 cfu/ mL, which were above the relevant standards. Salmonella counts were the highest at 3.1 to 3.9 × 103cfu/mL, with a prevalence of 40%. Considering its high prevalence, the virulence of Salmonella spp. was studied. The invA gene was detected in 35% of the Salmonella isolates by polymerase chain reaction (PCR). The findings suggested that pathogens adapt to this habitat, resulting in contamination of the indigenous fauna.
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    Prevalence, Serogroups, Shiga-toxin Genes and Pulsed Field Gel Electrophoresis Analyses of Escherichia coli Isolated from Bovine Milk
    (Springer Nature, 2013) D’Costa, Dilecta; Bhosle, Saroj N.; Dhuri, R. B.; Doijad, S. P.; Poharkar, K. V.; Kalorey, D. R.; Barbuddhe, S. B.
    Shiga toxin-producing Escherichia coli (STEC) including non-O157 strains have been linked to outbreaks and sporadic cases of illness worldwide. A total of 647 milk samples were collected at different levels of collection and processing (udder, milking utensils, milk collection centres and receiving dock) within West Coast region of India. The milk samples were screened for the presence of E. coli and further tested for the Shiga-toxin (stx) genes by PCR. The isolates were characterized for their serogroups and XbaI digestion patterns of total DNA separated by pulsed-field gel electrophoresis (PFGE). A total of 77 (11.90 %) isolates were confirmed as having E. coli. The serogroups reported were O4, O60, O112, O56, O159, O120, O2, O83, O88, O95, O141, O21, O25, O80, O140, O97, O24, O166, O146, O51, O169, O147, O103, O18, O100, O15, O69, O43, O7, O3, O45, O124, O110, O84, and O114. Out of the 77 E. coli isolates, 25 (32.46 %) could be classified as Shiga-toxigenic based on PCR results. Of these 11, 3 and 11 isolates were positive for stx1, stx2, and both stx1 and stx2, respectively. PFGE profiles indicated genetic diversity of E. coli strains. Much variation was observed among isolates recovered at different levels of collection. Further research is needed to uncover unique characteristics and resistance of non-O157 STEC strains.