Browsing by Author "Poharkar, Krupali V."

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    Characterization of Listeria monocytogenes isolated from human clinical cases
    (International Journal of Medical and Health Sciences (IJMHS), 2015) Kalekar, Sushanta; Doijad, Swapnil; Poharkar, Krupali V.; Rodriguez, Savio; Kalorey, D R; Kurkure, Nitin V; Rawool, Deepak B; D'Costa, Dilecta; Bhosle, Saroj; Barbuddhe, S B
    Introduction: Listeria monocytogenes is an emerging foodborne pathogen and causative agent of listeriosis. It is one of the cause of spontaneous abortions and meningitis. The objective of the study was to determine the incidences of L. monocytogenes from human cases of spontaneous abortions or having a history of spontaneous abortions and meningitis. Materials and Methods: A total of 481 samples from humans having a history of spontaneous abortions and meningitis were analyzed. Isolation of the pathogen was attempted employing two step enrichment followed by plating on selective media. Characterization of the isolates was based on biochemical tests, haemolysis on blood agar, CAMP test, phosphatidyl inositol-speciï¬ c phospholipase C assay, multiplex PCR to detect virulence-associated genes (hlyA, actA and iap) and serotyping by conventional and PCR based method. Results: Of the 481 samples analysed, 7 (1.45%) samples from abortion cases were found positive, while no CSF sample from meningitis cases was positive for L. monocytogenes. All the isolates showed in-vitro virulence characteristics such as hemolysis on 5% sheep blood agar, positive CAMP test and showed hallow formation on ALOA medium. All the isolates showed the presence of the hly, actA and iap genes. Conventional serotyping as well as PCR based serotyping revealed the isolates to be of 4b, 1/2b and 4e serotypes. Antibiotic sensitivity assay showed that the isolates were sensitive to trimethoprim, however, the isolates were least sensitive to meropenem. Conclusions: The study highlights the incidence of L. monocytogenes in humans with spontaneous abortions. KEYWORDS: Listeria monocytogenes, Serotypes, Isolation, PCR, human.
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    Mangrove ecosystems: An adopted habitat for pathogenic Salmonella spp.
    (Water Environment Federation, 2016) Poharkar, Krupali V.; Kerkar, Savita; D’Costa, Dilecta; Doijad, Swapnil; Barbuddhe, S. B.
    Mangroves are affected by industrial and anthropogenic factors. Although mangroves have been widely studied, investigations of pathogens that may affect public health significance are largely lacking even while incidences of diseases linked with the consumption of mangrove-associated food have increased. A total of 150 samples of water, sediment, and biota were collected from ten mangrove ecosystems in Goa, India. Total viable counts of pathogens such as E. coli, Listeria, Salmonella, and Vibrio spp. ranged from 1.25 to 3.9 × 103 cfu/ mL, which were above the relevant standards. Salmonella counts were the highest at 3.1 to 3.9 × 103cfu/mL, with a prevalence of 40%. Considering its high prevalence, the virulence of Salmonella spp. was studied. The invA gene was detected in 35% of the Salmonella isolates by polymerase chain reaction (PCR). The findings suggested that pathogens adapt to this habitat, resulting in contamination of the indigenous fauna.