Govt. College of Arts,Science & Commerce, Khandola, Marcela-Goa
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Mangrove ecosystems: An adopted habitat for pathogenic Salmonella spp.
(Water Environment Federation, 2016) Poharkar, Krupali V.; Kerkar, Savita; D’Costa, Dilecta; Doijad, Swapnil; Barbuddhe, S. B.
Mangroves are affected by industrial and anthropogenic factors. Although mangroves have been widely studied, investigations of pathogens that may affect public health significance are largely lacking even while incidences of diseases linked with the consumption of mangrove-associated food have increased. A total of 150 samples of water, sediment, and biota were collected from ten mangrove ecosystems in Goa, India. Total viable counts of pathogens such as E. coli, Listeria, Salmonella, and Vibrio spp. ranged from 1.25 to 3.9 × 103 cfu/ mL, which were above the relevant standards. Salmonella counts were the highest at 3.1 to 3.9 × 103cfu/mL, with a prevalence of 40%. Considering its high prevalence, the virulence of Salmonella spp. was studied. The invA gene was detected in 35% of the Salmonella isolates by polymerase chain reaction (PCR). The findings suggested that pathogens adapt to this habitat, resulting in contamination of the indigenous fauna.
Transformation of alpha‐santonin via two independent pathways by Pseudomonas strain S ATCC 43388
(Oxford University Press, 1993) Colaco, Dilecta; Furtado, I.; Naik, U. P.; Mavinkurve, S.; Paknikar, S. K.
Isolation and Characterization of Listeria Species from Raw and Processed Meats
(Indian Association of Veterinary Public Health Specialists, 2010) Doijad, S.P.; Vaidya, V.; Garg, S.; Kalekar, S.; Rodrigues, J.; D’Costa, Dilecta; Bhosle, S. N.; Barhuddhe, S. B.
A total of 109 meat and meat product samples (50 beef, 52 pork sausages and 7 pork) were examined for the presence of Listeria species. Isolates were confirmed by biochemical characterization and haemolysis on blood agar. A total of 25 (22.94 percent) isolates were recovered. Of these, 7 (6.42 percent) were L. monocytogenes, while one (0.9 percent) was L. ivanovii. Other isolates were L. seeligeri (10), L. innocua (4), L. welshimeri (2) and L. grayi (1). All the isolates were further subjected to a PCR assay for detection of the hlyA gene, the hlyA gene was detected in 7 isolates. Multiplex-PCR serotyping assay revealed three of the strains belonging to serovar group 4b, 4d, 4e, and four to serovar group 1/2a, 3a. The prevalence of L. monocytogenes in raw meat, particularly serogroup 4b, 4d, 4e in present study indicates a potential threat to public health.
Bacteriological Quality of Milk at Different Levels of Collection in Goa
(Indian Association of Veterinary Public Health Specialists, 2011) D’Costa, Dilecta; Bhosle, S. N.; Dhuri, R. B.; Barbuddhe, S. B.
The bacteriological quality of milk at different levels of collection was studied. Samples were collected in sterile containers at quarterly intervals. The samples were analysed for total vibal count, methylene blue reduction test (MBRT) and California mastitis test (CMT). Subclinical mastitis was found in 23.8 percent of the animals. The average methylene blue reduction time decreased from the farm to the processing unit. The average counts were 2.67x104, 1.59x104, 9.23x105 and 2.35x106 cfu/ml at farmers' field, milking utensils, collection centers and receiving dock (processing point), respectively. The milking methods, milk containers and time interval from the collection at the udder level to the receiving dock played a major role in the deterioration of the milk quality along with the climatic conditions prevalent in a particular season.
Prevalence, Serogroups, Shiga-toxin Genes and Pulsed Field Gel Electrophoresis Analyses of Escherichia coli Isolated from Bovine Milk
(Springer Nature, 2013) D’Costa, Dilecta; Bhosle, Saroj N.; Dhuri, R. B.; Doijad, S. P.; Poharkar, K. V.; Kalorey, D. R.; Barbuddhe, S. B.
Shiga toxin-producing Escherichia coli (STEC) including non-O157 strains have been linked to outbreaks and sporadic cases of illness worldwide. A total of 647 milk samples were collected at different levels of collection and processing (udder, milking utensils, milk collection centres and receiving dock) within West Coast region of India. The milk samples were screened for the presence of E. coli and further tested for the Shiga-toxin (stx) genes by PCR. The isolates were characterized for their serogroups and XbaI digestion patterns of total DNA separated by pulsed-field gel electrophoresis (PFGE). A total of 77 (11.90 %) isolates were confirmed as having E. coli. The serogroups reported were O4, O60, O112, O56, O159, O120, O2, O83, O88, O95, O141, O21, O25, O80, O140, O97, O24, O166, O146, O51, O169, O147, O103, O18, O100, O15, O69, O43, O7, O3, O45, O124, O110, O84, and O114. Out of the 77 E. coli isolates, 25 (32.46 %) could be classified as Shiga-toxigenic based on PCR results. Of these 11, 3 and 11 isolates were positive for stx1, stx2, and both stx1 and stx2, respectively. PFGE profiles indicated genetic diversity of E. coli strains. Much variation was observed among isolates recovered at different levels of collection. Further research is needed to uncover unique characteristics and resistance of non-O157 STEC strains.