Rai, Anupkumar J.D’Costa, Dilecta2024-12-302024-12-302024Indian Journal of Natural Sciences. 14(82); 2024; 68322-68327.0976-0997https://tnsroindia.org.in/JOURNAL/issue82/IJONS%20-%20ISSUE%2082%20-%20%20FEB%202024%20-%20FULL%20TEXT%20PART%20%20-%2001.pdfhttp://khandolacollege.ndl.gov.in/handle/123456789/132The availability of drinking water is one of the primary passenger amenities provided by the railways. The present study was undertaken to investigate and evaluate the water quality of the trains plying on the Konkan railway route. The physicochemical and microbiological studies were carried out to determine the potability of water supplied by railways. The isolation and phenotypic characterization of various pathogens were mainly emphasized. All the analyzed samples showed the presence of coliforms and other organisms indicative of fecal contamination. Seasonal analysis of microbial load indicated that monsoon favours the growth and proliferation of microbes. The biochemical assessment showed the presence of several pathogens like E. coli, Salmonella spp., Vibrio spp., and Clostridium spp. This is a matter of public health concern to train commuters which in turn calls for better sanitary measures.enNATURAL SCIENCES::Biology::Organism biology::MicrobiologyArticle